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Serology

ANA Immunofluorescent
(ANA-IMF)

TEST    :    ANA Immunofluorescent

OTHER TEST REQUEST NAME:
• Fluorescent Antinuclear Antibody
• ANA with dilution and pattern

TEST COMPOSITION:
Not Applicable

INTENDED USE:
Aids in the diagnosis of autoimmune disorders which helps to determine the progression of the disease. Most often used in the diagnosis of Systemic Lupus Erythematosus (SLE)

METHODOLOGY:
Indirect Immunofluorescence

LABORATORY SECTION:
Molecular Diagnostic Section

SPECIAL INSTRUCTIONS / PATIENT PREPARATIONS:
No patient preparation required.

COLLECTION/SAMPLE CONTAINER:
Red tube (DO NOT USE GOLD TUBE or any other type of tube with gel separator)

SPECIMEN AND VOLUME REQUIREMENT
Note: Follow tube manufacturer recommendation.    :
2-3mL Serum

ALTERNATIVE SPECIMEN AND VOLUME REQUIREMENT:    
Not Applicable

SPECIMEN STABILITY
•   ROOM TEMPERATURE (15-25°C):     Not Specified
•   REFRIGERATED TEMPERATURE (2-8°C):    7 Days
•   FREEZER TEMPERATURE (-20°C)    :    30 Days

TRANSPORT TEMPERATURE:
Transport specimen at 2 – 8 °C (with cold packs)

REJECTION CRITERIA:
•  Gold top tube (Collection tube WITH gel separator)
•  EDTA (Collection tube WITH gel separator)
•  With signs of bacterial contamination
•  Markedly lipemic serum
•  Improperly labeled specimens
•  Hemolyzed specimens
•  Quantity not sufficient
•  Specimens exceeded sample stability requirement

RUNNING DAY:
Thursday

CUT OFF TIME:
Wednesday, 6:00PM

TAT/RELEASING OF RESULTS:
Friday, 2:00 PM

REFERENCE INTERVAL / RESULT INTERPRETATION:
Pattern: No Pattern
Result Interpretation:

Adult :
Negative:  <80

Children <16 y/o :
Negative : <40

Pattern: Homogenous, Speckled, Nucleolar, Centromere, Cytoplasmic

 

Result interprepation:

Adult :
Positive threshold  :  80
Low Titer                  : 160-320
High Titer                 : >320

Children <16 y/o :
Titer of 40 maybe significant


 

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